Nanoencapsulated vitamin D is stable and bioavailable – May 2012

Stability and bioavailability of vitamin D nanoencapsulated in casein micelles.

Food Funct. 2012 May 9.
Haham M, Ish-Shalom S, Nodelman M, Duek I, Segal E, Kustanovich M, Livney YD.
Biotechnology and Food Engineering Department, The Technion, Israel Institute of Technology, Haifa, 32000, Israel. livney@technion.ac.il.

We have previously introduced the potential of casein micelles (CM) as natural nanovehicles for hydrophobic nutraceuticals, e.g. vitamin D (VD) (E. Semo, E. Kesselman, D. Danino and Y. D. Livney, Food Hydrocolloids, 2007, 21, 936-942). The aims of the current study were to improve performance by adding an ultra-high-pressure homogenization step, and to evaluate the protection conferred by re-assembled CM (rCM) against VD thermal and oxidative degradation, and the bioavailability of VD(3) in rCM, by a clinical trial. Dynamic-light-scattering showed that VD(3)-rCM had a diameter of 91 ± 8 nm (average ± standard error). When VD(3) was encapsulated in rCM and subjected to thermal treatment (80 °C, 1 min), no significant loss was observed (P > 0.05), compared to 13 and 14% loss of VD(3) emulsified with Tween-80 (a synthetic emulsifier typically used for VD solubilization) and of unencapsulated VD(3) respectively (P < 0.05). VD(3) in rCM was also more stable during 28 d cold storage (?40% loss) compared to Tween-80 emulsified (?50% loss) or to un-encapsulated (?70% loss) VD(3). Ultra-high-pressure homogenization of VD(3)-rCM (?155 MPa) significantly enhanced vitamin stability, resulting in only ?10% loss after 28 d of storage.

Bioavailability of a single-dose of 50?000 international-units (IU) VD(3) encapsulated in rCM, in 1% fat milk, investigated in a randomized double blinded placebo controlled clinical study with 87 human volunteers, was at least as high as that using an aqueous Tween-80-emulsified VD(3) supplement. We conclude that ultra-high-pressure homogenization treated rCM protect VD(3) against heat- and storage-induced degradation, and VD(3) encapsulated in rCM is highly bioavailable.
PMID: 22569895

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See also VitaminDWiki

• Forms of Vitamin D category listing has 113 items along with related searches
• Bio-emulsified drops doubled vitamin D levels – Dec 2010
• Vitamin D bioavailability: State of the art – Oct 2014
• Vitamin D Emulsions - nano 2X better than coarse – Dec 2017

See also web

• Food-grade nanoemulsions: formulation, fabrication, properties, performance . .
  April 2011, 45 pages, but abstract does not have much content