Vitamin D-binding Protein in Cerebrospinal Fluid is Associated with Multiple Sclerosis Progression.
Mol Neurobiol. 2013 Jan 22.
Yang M, Qin Z, Zhu Y, Li Y, Qin Y, Jing Y, Liu S.
Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China.
Multiple sclerosis is a neurological disorder that presents with symptoms including inflammation, neurodegeneration, and demyelination of the central nervous system (CNS).
Secondary progressive multiple sclerosis (SPMS) manifests with serious physical disability. To quantitatively analyze differential protein expression in patients with SPMS, we performed two-dimensional fluorescence difference in-gel electrophoresis, followed by mass spectrometry on the cerebrospinal fluid of these patients and patients with other neurological diseases.
Vitamin D-binding protein (DBP), gelsolin, albumin, etc. showed more than a 1.5-fold difference between the two groups. Based on these results, an experimental allergic encephalomyelitis (EAE) model of multiple sclerosis in Lewis rats was used to investigate DBP's role in the disease. Protein levels, mRNA transcripts, and ligands of DBP in different regions of the CNS were evaluated under various vitamin D intake levels.
Here, DBP levels increased in the experimental rat groups compared to the control groups regardless of vitamin D intake. Moreover, DBP mRNA levels varied in different parts of the CNS including spinal cords in the experimental groups. The observed differences between DBP protein and mRNA levels in the experimental groups' spinal cords could be derived from the disruption of the blood-brain barrier. Furthermore, an interaction between DBP and actin was confirmed using coimmunoprecipitation and western blot. These results indicate a role for DBP in the actin scavenge system.
Moreover, in the experimental group that received oral vitamin D3 supplement, we observed both delayed onset and diminished severity of the disease.
When DBP was upregulated, however, the benefits from the vitamin D3 supplements were lost. Thus, we inferred that high levels of DBP were adverse to recovery.
In conclusion, here we observed upregulated DBP in the cerebrospinal fluid could serve as a specific diagnostic biomarker for the progression of multiple sclerosis. Next, we demonstrate the vital function of increased levels of free vitamin D metabolites for multiple sclerosis treatment. Finally, vitamin D supplements may be particularly beneficial for SPMS patients.
PMID: 23339019
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See also VitaminDWiki
- Vitamin D prevents multiple sclerosis through multiple mechanisms – Jan 2013
- Overview MS and vitamin D
- includes Controversy: Which is better: UV or Vitamin D
- Vitamin D can both prevent and treat Multiple Sclerosis – review Nov 2012
- Hypothesis of Autoimmunity which includes Epstein-Barr Virus and Vitamin D Deficiency – 2012
- Epigenetics, vitamin D, and Multiple Sclerosis
- Clinical trials for MS and Vitamin D INTERVENTION 19 as of Jan 2013
- All items in category MS and vitamin D
412 items - Search VitaminDWiki for Gc-globulin OR "vitamin D-Binding" 1920 items as of Jan 2013
See also web
- About.com good overview of Secondary progressive MS
- WebMD – a few highlights follow
People with secondary progressive multiple sclerosis (SPMS) start out with another type of MS – - relapsing-remitting multiple sclerosis.
If you've been diagnosed with secondary progressive MS you may have had relapsing-remitting MS for a decade or more. That's when you may begin to experience a shift in your disease.
Most people with relapsing-remitting MS — about 80% — eventually develop secondary progressive MS. The relapses and remissions that used to come and go change into a steady, gradual worsening of the disease. The shift typically begins 15 to 20 years after first being diagnosed with MS.
Late-stage MS associated with protein in spinal cord which blocks vitamin D – Jan 2013(Cached) Printer Friendly Follow this page for updates9256 visitors, last modified 14 May, 2014, This page is in the following categories (# of items in each category)